Gene description for GNAI1
Gene name G protein subunit alpha i1
Gene symbol GNAI1
Other names/aliases GBI1
Species Sus scrofa
 Experiment description of studies that identified GNAI1 in Polarised retinal pigmented epithelial cells

1
Experiment ID917
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - apical - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

2
Experiment ID918
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - basolateral - Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

3
Experiment ID919
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - basolateral - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

4
Experiment ID920
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - apical - Fraction 9
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

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